Online Inquiry
FVB-Tg(GFAP-cre)25Mes/J
Cat. No.: CEMM-07250018
Homozygotes for this transgene are not viable. Hemizygotes express Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target, and recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. This mutant mouse strain represents an effective tool for generating central nervous system specific-targeted mutants.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(GFAP-cre)25Mes
show more
close
|
| Allele Name |
transgene insertion 25
show more
close
|
| Allele Attributes |
Recombinase-expressing
show more
close
|
| Gene Symbol |
Tg(GFAP-cre)25Mes
show more
close
|
| Gene Name |
transgene insertion 25
show more
close
|
| Chromosome |
UN
show more
close
|
| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
Primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons; also periportal cells of the liver
show more
close
|
| Strain of Origin |
FVB/N
show more
close
|
| Molecular Note |
This transgene expresses Cre recombinase under the control of a human glial fibrillary acidic protein (GFAP) promoter. Cre-mediated recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. Expression activity is also present in periportal cells of the liver. Developmental onset of transgene expression occurs in the dorsal and medial regions of the telencephalon by embryonic day 13.5. In adult cerebellum, only astrocytes are immunoreactive for GFAP or Cre recombinase.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
FVB/NJ Noncarrier
show more
close
|
| Breeding Considerations |
The strain is maintained as a hemizygote on the FVB/N background. For Cre-lox experiments, researchers are urged to monitor their Cre;floxed double mutant mice for any undesirable germline deletion of the floxed allele (e.g. genotyping tail/ear samples). Researchers may consider also maintaining a GFAP-Cre colony separate from any floxed colonies.
show more
close
|
| Breeding Strategy |
Noncarrier x Hemizygote; Hemizygote x Noncarrier
show more
close
|
For Research Use Only.
Related Products