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129S.Cg-Ndor1Tg(UBC-cre/ERT2)1Ejb/J
Cat. No.: CEMM-07250062
Common Name: UBC-Cre-ERT2
When these UBC-Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in widespread cells/tissues. In Goodwin et al. 2019 Genome Res. 29:494, it was discovered that the transgene integrated into chromosome 2 causing a 5 bp deletion in the NADPH dependent diflavin oxidoreductase 1 locus (Ndor1).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Ndor1Tg(UBC-cre/ERT2)1Ejb
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| Allele Name |
transgene insertion 1
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| Allele Attributes |
Recombinase-expressing; Inducible
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| Gene Symbol |
Ndor1
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| Gene Name |
NADPH dependent diflavin oxidoreductase 1
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| Chromosome |
2
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| Expressed Genes |
ESR1, estrogen receptor 1, human; Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Tamoxifen-inducible Cre activity in all tested tissues
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| Strain of Origin |
(129S/SvEv x C57BL/6)
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| Molecular Note |
This Cre-ERT2 transgenic mouse line was generated via lentitransgenesis; a self-inactivating lentiviral vector was designed with the human ubiquitin C (UBC) promoter sequence upstream of a Cre-ERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Cre-ERT2-expressing lentivirus were microinjected into the perivitelline space of one-cell zygotes (129SvEv;C57BL/6 mixed genetic background). Mice display tamoxifen-inducible Cre activity in all tissue types. Line 1 inserted into the gene at 25249816-25249821 (Build GRCm38/mm10) resulting in a 5 bp deletion.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
129S1/SvImJ
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| Breeding Considerations |
When maintaining a live colony, transgenic mice can be bred to wildtype siblings or to (129S1/SvImJ). A homozygous phenotype is not yet characterized (Aug-2007).
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For Research Use Only.
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