D2.Cg-Tg(Thy1-Brainbow1.1)MLich/SjJ

Cat. No.: CEMM-07250614

These Brainbow 1.1 (founder line M) mice allow mutually exclusive expression of three membrane-targeted fluorescent proteins in astrocytes (including glia) throughout the brain, providing unambiguous delineation of boundaries between adjacent astrocytes in cre recombined cells, and may also be useful in conjunction with other Brainbow strains for neurobiological studies.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Thy1-Brainbow1.1)MLich show more close
Allele Name	transgene insertion M show more close
Allele Attributes	Reporter show more close
Gene Symbol	Tg(Thy1-Brainbow1.1)MLich show more close
Gene Name	transgene insertion M show more close
Chromosome	UN show more close
Expressed Genes	RFP, Red Fluorescent Protein, coral; YFP, Yellow Fluorescent Protein, jellyfish; OFP, Orange Fluorescent Protein, jelly fish; CFP, Cyan Fluorescent Protein, jellyfish show more close
MGI Accession ID	3761292 show more close
Site of Expression	When bred to Cre recombinase expressing mice, there is one of three expression outcomes for each transgene copy in each cell of the Cre expressing tissue(s): mCerulean (CFP), mYFP, or mCherry (RFP). Integration of multiple tandem transgene copies allows for labeling of individual astrocytes of all areas of the brain and spinal cord, as well as dentate gyrus granule cells. show more close
Strain of Origin	C57BL/6 x CBA show more close
Molecular Note	The Thy1-Brainbow 1.1 transgene was designed with the mouse Thy1 regulatory elements surrounding four fluorescent proteins (orange (OFP), red (RFP), enhanced yellow (EYFP), and cyan (CFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.1 coding region contained (from 5' to 3') a LoxN site, Lox2272 site, LoxP site, Kusabira-Orange OFP (kOFP; with polyA sequence), LoxN site, mCherry RFP (with membrane tethering palmitoylation sequence and polyA sequence), Lox2272 site, monomeric EYFP (mYFPA206K; with membrane tethering palmitoylation sequence and polyA sequence), LoxP site, mCerulean CFP (with membrane tethering palmitoylation sequence and 5? FRT site and polyA sequence).Kusabira-Orange (KO or kOFP) is a true OFP with an artificial amino acid sequence attached to the N-terminus of the protein allowing bright orange fluorescence in cells. The mCherry RFP is an optimized DsRed/mRFP1 fluorescent protein variant with a combination of amino acid substitutions designed to improve spectral properties. The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization. show more close

HUSBANDRY
Breeding Considerations	Hemizygotes are viable and fertile. show more close

For Research Use Only.