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D2.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato, -EGFP)Luo/SjJ
Cat. No.: CEMM-07250616
ROSAmT/mG is a cell membrane-targeted, two-color fluorescent Cre reporter allele; expressing cell membrane-localized red fluorescence in widespread cells/tissues prior to Cre recombinase exposure, and cell membrane-localized green fluorescence in Cre recombinase expressing cells (and future cell lineages derived from these cells). These ROSAmT/mG mice function similarly to ROSAnT-nG mice, with ROSAmT/mG directed to cell membrane and ROSAnT-nG directed to nuclei.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo
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| Allele Name |
targeted mutation 4
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
RFP, Red Fluorescent Protein, coral; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Membrane-targeted tdTomato (mT) is expressed in all tissues and cell types examined. When bred to Cre recombinase expressing mice, the mT cassette is deleted in the Cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG).
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
The targeting vector was designed with a CMV enhancer/chicken beta-actin core promoter (pCA) driving expression of a loxP-flanked, N-terminal membrane-tagged, optimized DsRed fluorescent protein variant (called tandem-dimer-Tomato or tdTomato) sequence followed by a polyadenylation signal. Immediately distal to the second loxP site is an N-terminal membrane-tagged, enhanced green fluorescent protein (EGFP) sequence itself followed by a polyadenylation signal. An FRT flanked neo cassette was also located distal to the expression vector. Red fluorescence is detected in all tissues tested. When mice are bred to Cre expressing mice, the floxed region is excised in Cre-expressing tissue and this allows expression of the EGFP cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
DBA/2J
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| Breeding Considerations |
Heterozygotes and homozygotes are viable and fertile.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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