C57BL/6N-Sned1^{tm1a(KOMP)Wtsi}/HynMmjax

Sned1^{tm1a(KOMP)Wtsi}

targeted mutation 1a

Conditional ready (e.g. floxed); Reporter; Null/Knockout

Sned1

sushi

1

4362972

C57BL/6N

The L1L2_Bact_P cassette was inserted at position 93186212 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 93187769. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be Created by flp recombinase expression in mice carrying this allele. Subsequent Cre expression results in a knockout mouse. If Cre expression occurs without flp expression, a reporter knockout mouse will be Created.

C57BL/6NJ

Heterozygotes are viable and fertile. Most homozygotes die within 2 days of being born. The rare knock-out survivors present severe craniofacial defects (shorter snouts, asymmetrical nasal cavities) and growth retardation.

Wild-type x Heterozygote; Heterozygote x Wild-type