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B6;129-Igs1tm10(CAG-Bgeo, -Fzd6)Nat/J
Cat. No.: CEMM-07250518
This knock-in, 2 kb 5' of the Ubb (ubiquitin B) gene, gives constitutive expression of a nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo) in the absence of cre-mediated recombination. Following cre-mediated recombination, the NLS-beta-geo coding region is excised and the downstream triple HA epitope (3HA) tagged Fzd6 (frizzled homolog 6 (Drosophila)) coding region is expressed.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs1tm10(CAG-Bgeo,-Fzd6)Nat
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| Allele Name |
targeted mutation 10
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| Allele Attributes |
Conditional ready (e.g. floxed); Inserted expressed sequence; No functional change
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| Gene Symbol |
Igs1
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| Gene Name |
intergenic site 1
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| Chromosome |
11
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| Expressed Genes |
HA tag, HA tag; Fzd6, frizzled class receptor 6, mouse, laboratory
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| MGI Accession ID | show more close |
| Site of Expression |
The HA tagged Fzd6 can be activated by Cre recombinase in any cell in the body at any time in development following Cre-mediated recombination.
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
The following elements were inserted, in order into a region 2 kb 5' of the Ubb gene by targetedrecombination: CMV enhancer/beta-actin promoter, loxP site, beta-geo, 3 stop transcription sites, loxP site, 3HA-Fzd6, rabbit beta-globin 3' UTR. The 3HA tag is inserted after the signal peptide and before the cysteine-rich domain of Fzd6.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
Heterozygotes are viable and fertile, but homozygotes are unhealthy.
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For Research Use Only.
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