Online Inquiry
C57BL/6J-Tg(CAG-lacZ, -FUS, -EGFP)629Gyu/J
Cat. No.: CEMM-07250646
Common Name: CAG-Z-FUSWT-IRES-EGFP transgenic line 629 [CAG-Z-FUSWT (629)]
The CAG-Z-FUSWT-IRES-EGFP transgenic line 629 [or CAG-Z-FUSWT (629)] has widespread expression of lacZ, that is replaced by expression of wildtype human fused in sarcoma protein (huFUSWT) and EGFP fluorescence following exposure to Cre recombinase. The Cre recombinase-inducible expression of huFUSWT, along with similarly designed transgenic mice harboring the FUSR521G mutant isoform, is useful in studying FUS-linked juvenile amyotrophic lateral sclerosis (ALS; Lou Gehrig's Disease), and the neurodegenerative disorder frontotemporal lobar degeneration (FTLD).
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(CAG-lacZ,-FUS,-EGFP)629Gyu
show more
close
|
| Allele Name |
transgene insertion 629
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence; Humanized sequence
show more
close
|
| Gene Symbol |
Tg(CAG-lacZ,-FUS,-EGFP)629Gyu
show more
close
|
| Gene Name |
transgene insertion 629
show more
close
|
| Chromosome |
UN
show more
close
|
| Expressed Genes |
lacZ, beta-galactosidase, E. coli; FUS, fused in sarcoma, human; GFP, Green Fluorescent Protein
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
Prior to Cre recombination LacZ is widely expressed; the human wildtype fused in sarcoma protein and EGFP are expressed only after Cre recombination in Cre-expressing tissues.
show more
close
|
| Strain of Origin |
C57BL/6J
show more
close
|
| Molecular Note |
This transgene consists of the cytomegalovirus immediate early enhancer-chicken beta-actin hybrid (CAG) promoter, a floxed lacZ gene, wild-type human FUS cDNA, IRES, and EGFP coding sequences. Cre-excision of the loxP flanked lacZ DNA sequence allows for translation of FUS and EGFP. Two lines were generated: 629 and 638. Line 629 was chosen as a representative line.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
show more
close
|
| Breeding Considerations |
When maintaining a live colony, transgenic mice may be bred with wildtype (non-carrier) mice from the colony or with C57BL/6J inbred mice. it has not been attempted to make this strain homozygous.
show more
close
|
For Research Use Only.
Related Products