B6.Cg-Igs7^{tm140.1(tetO-EGFP, CAG-tTA2)Hze}/J

Igs7^{tm140.1(tetO-EGFP,CAG-tTA2)Hze}

targeted mutation 140.1

Conditional ready (e.g. floxed); Reporter; Inducible; Transactivator

Igs7

intergenic site 7

9

GFP, Green Fluorescent Protein; tTA, tetracycline-controlled transactivator, E. coli

5904001

When bred to mice expressing Cre recombinase, the resulting offspring will have both floxed-STOP cassettes deleted in the Cre-expressing cells/tissues - resulting in EGFP fluorescence and tTA2 expression.

(129S6/SvEvTac x C57BL/6NCrl)F1

The replacement vector is designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element, a Tet response element/promoter (TRE2), a loxP-flanked STOP cassette, a synthetic enhanced green fluorescent protein sequence (EGFP), a woodchuck hepatitis virus post-transcriptional regulatory element, a BGH polyA, two copies of chicken beta-globin HS4 insulator element, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from pCAGGS), a lox2272-flanked STOP cassette, a synthetic modified tetracycline-regulated transactivator gene (tTA2S), a WPRE, a BGH polyA, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site. Embryonic stem cells previously targeted with FRT3::AttB::PGK-neoR-polyA::FRT5::splice acceptor::3'hygro cassette::SV40 polyA:AttP in TIGRE, were re-transfected with the pTRE2-LSL-EGFP-CAG-LSL-tTA2 replacement vector and Flp recombinase vector for recombinase-mediated cassette exchange (RCME). PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL).

C57BL/6J

Heterozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. It is the experience of CD BioScience that homozygous mice are viable and fertile. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice may be bred together.

Homozygote x Homozygote