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B6J.129S6(FVB)-Slc32a1tm2(cre)Lowl/MwarJ
Cat. No.: CEMM-07250701
Common Name: Vgat-ires-cre knock-in (C57BL/6J)
Vgat-ires-Cre knock-in mice have Cre recombinase expression directed to inhibitory GABAergic neuron cell bodies, without disrupting endogenous vesicular inhibitory amino acid transporter expression. These mice may be used to generate conditional mutations for studying gain-or-loss of function and/or fate mapping related to GABAergic neuronal function.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Slc32a1tm2(cre)Lowl
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Slc32a1
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| Gene Name |
solute carrier family 32 (GABA vesicular transporter)
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| Chromosome |
2
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Cre recombinase activity is detected in inhibitory GABAergic neuron cell bodies in the caudate putamen, suprachiasmatic nucleus, central amygdaloid nucleus, and zona incerta, nucleus accumbens, lateral septum, medial septum, reticular nucleus of the thalamus, substantia nigra pars reticulata, and Purkinje cell layer of the cerebellum.
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| Strain of Origin |
129S6/SvEvTac
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| Molecular Note |
A targeting vector, constructed from a BAC clone containing the Slc32a1 (Vgat) gene, was utilized to insert an internal ribosomal entry site followed by the Cre recombinase sequence into the Slc32a1 locus just downstream of the endogenous stop codon. Cre is expressed from the Slc32a1 gene promoter. Immunohistochemistry on brain and Western blot analysis shows deCreased expression of SLC32A1 protein in homozygotes.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Both heterozygous and homozygous mice are viable and fertile with no gross physical abnormalities. When maintaining a live colony Repository, homozygous mice may be bred together.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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