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C57BL/6-Gt(ROSA)26Sortm9(Rac1*, EGFP)Rsky/J
Cat. No.: CEMM-07250220
Common Name: Rosa26-LSL-Rac1G12V (or R26STOPFLRACDA)
The R26StopFLRACDA conditional allele is targeted to the Gt(ROSA)26Sor locus and has a loxP-flanked Neo-STOP cassette preventing transcription of the downstream bicistronic sequences (RACDA [RacG12V; a mutant form of Rac1 rendered constitutively active by a glycine->valine substitution at amino acid 12] and EGFP). These R26StopFLRACDA mice allow inducible constitutive activity of Rac1-dependent signal transduction; which is associated with gene expression, proliferation, cell survival, cell cycle progression, cytoskeletal reorganization, and Rho- and CDC42-pathways.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm9(Rac1*,EGFP)Rsky
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| Allele Name |
targeted mutation 9
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Constitutively active; Inserted expressed sequence
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Cre excision of the stop signal results in expression of EGFP and a constitutively active form of Rac1 (RACDA) in Cre-expressing tissues.
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| Strain of Origin |
C57BL/6
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| Molecular Note |
A targeting vector designed with (from 5' to 3') a splice acceptor site, a loxP-flanked Neo-STOP cassette, a cDNA sequence encoding RACDA (RacG12V; a mutant form of Rac1 rendered constitutively active by a glycine->valine substitution at amino acid 12]), an FRT flanked internal ribosomal entry site-enhanced green fluorescent protein cassette (IRES-EGFP), and bovine polyadenylation signal. This transgene construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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