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B6.Cg-Adcyap1r1tm1.1(cre/GFP)Rpa/J
Cat. No.: CEMM-07251027
Common Name: Adcyap1r1iCre:GFP
Adcyap1r1iCre:GFP knock-in mice should express iCre:GFP wherever Adcyap1r1 (adenylate cyclase activating polypeptide 1 receptor 1), a G protein-coupled receptor (GPCR), is normally expressed. Expression levels of the targeted gene have not been assessed, but no effect is anticipated as the insertion is beyond the termination codon.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Adcyap1r1tm1.1(cre/GFP)Rpa
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| Allele Name |
targeted mutation 1.1
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| Allele Attributes |
Recombinase-expressing; Reporter
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| Gene Symbol |
Adcyap1r1
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| Gene Name |
adenylate cyclase activating polypeptide 1 receptor 1
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
A cassette encoding IRES-mnCre:GFP (an internal ribosome entry sequence (IRES), a Myc-tag and nuclear localization signals at the N-terminus of Cre recombinase, which is fused to green fluorescent protein followed by a SV40 polyadenylation sequence) was inserted just 3' of the termination codon in the last coding exon of the Adcyap1r1 gene via homologous recombination. The neomycin cassette was excised through crosses with animals broadly expressing Flp recombinase.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Heterozygotes and homozygotes are viable and fertile.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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