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C57BL/6-Gt(ROSA)26Sortm1(CAG-ECFP*/Rapgef3/Venus*)Kama/J
Cat. No.: CEMM-07250907
Common Name: CAMPER; cAMP Encoded Reporter
When these CAMPER, cAMP Encoded Reporter, mice are crossed with a Cre recombinase expressing strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor and CAG promoters drive expression of the TEpacVV cAMP sensor in the Cre-expressing cells. CAMPER mice allow selective cAMP concentration changes monitoring via FRET imaging in a tissue/cell-specific manner and may be useful in studies of G-protein-coupled receptors (GPCRs) responses.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-ECFP*/Rapgef3/Venus*)Kama
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
C57BL/6NTac
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| Molecular Note |
The targeting vector contains from (5' to 3'): CAG promoter, a loxP STOP loxP cassette, Epac cAMP sensor, WPRE, polyadenylation signal sequence and is inserted between exons 1 and 2 of the Gt(ROSA)26Sor Ai9 targeting vector. The Epac sensor reports cAMP binding by changes in fluorescence energy transfer (FRET). The sensor consists of an mTurqoise donor, a monomeric variant of the cyan fluorescent protein, and a circularly permutated Venus enhanced yellow fluorescent protein, Venus-Venus, acceptor sandwiching the cAMP-binding protein Rapgef3.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
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| Breeding Considerations |
When maintaining a live colony, these mice can be bred as homozygotes.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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