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C57BL/6-Gt(ROSA)26Sorem1(CAG-Kcnq2*/EGFP)Hjch/J
Cat. No.: CEMM-07251124
Common Name: Kcnq2-M547Vfl
Kcnq2‐M547Vfl mice carry a floxed‐STOP cassette upstream of a Kcnq2 M547V‐ires‐EGFP cassette placed in the Gt(ROSA)26Sor gene. Upon excision of the floxed‐STOP cassette by Cre recombinase, this line expresses murine Kcnq2 gene containing the epileptic encephalopathy mutation M547V and enhanced green fluorescent protein (EGFP).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sorem1(CAG-Kcnq2*/EGFP)Hjch
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| Allele Name |
endonuclease-mediated mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
C57BL/6J
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| Molecular Note |
A CAG-loxP-STOP-loxP-Kcnq2 (M547V; ATG to GTG) cDNA-IRES-EGFP-polyA cassette is introduced into intron 1 in reverse transcriptional orientation by CRISPR/Cas9 methodologies.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J Wild-type from the colony
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| Breeding Considerations |
Heterozygous and homozygous floxed mice are viable and fertile.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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