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B6N.129P2(Cg)-Igs13tm1Dolm Igs14tm1Dolm/J

Cat. No.: CEMM-07250561

Common Name: 16p11flx
A copy number variation on human chromosome 16p11.2 is among the most common genetic variations found in autism spectrum disorders. 16p11flx mice are a Cre or FLP recombinase-inducible mouse model of 16p11.2 deletion that has several loxP and frt sites flanking the corresponding 440 kbp region on mouse chromosome 7F3. These mice may be useful in studying basal ganglia circuitry and the pathophysiology of autism.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Igs13tm1Dolm
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Allele Name
targeted mutation 1
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Allele Attributes
Conditional ready (e.g. floxed); No functional change
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Gene Symbol
Igs13
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Gene Name
intergenic site 13
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Chromosome
7
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MGI Accession ID
5569502
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Site of Expression
Following Cre or FLP-mediated deletion of the 440 kbp region on mouse chromosome 7F3region, a membrane-targeted fluorescent reporter gene (mCherry) is expressed in Cre or FLP expressing tissues.
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Strain of Origin
129P2/OlaHsd
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Molecular Note
The targeting vector is designed to insert a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), loxP-FRT neomycin resistance cassette-loxP-FRT loxP sequence and polyA signal at the centromeric end of the coronin actin binding protein 1A locus (Coro1a) gene. The insertion site is 1165 bp centromeric (transcriptionally downstream) of the last exon.
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HUSBANDRY
Suggested Controls
C57BL/6NJ
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Breeding Considerations
When maintaining a live colony, mice homozygous for each targeted allele may be bred together. Additionally, breeding heterozygous mice together results in mice that are black, light chinchilla or albino. While the Portmann et al. 2014 Cell Rep 7(4):1077-92 publication describes the mESC used were 129/OLA, the donating laboratory indicates it may have been a different 129 origin. Taken together, these data suggest the 16p11flx mice we received retained mESC-derived allele(s) at the tyrosinase locus (e.g. Tyrc and/or Tyrc-ch) that, because it is only ~40 kbp proximal on chromosome 7, appears to be segregating along with the 16p11flx region. Depending upon future segregation patterns, available mice may be black, light chinchilla or albino.
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For Research Use Only.
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