B6J.Cg-Tg(Mnx1-cre/ERT2)8Rbro/Mmjax

Cat. No.: CEMM-07251093

Hb9::CreERT2 transgenic mice express a tamoxifen inducible Cre recombinase/ERT2 fusion under the control of the mouse Mnx1 (also known as Hb9) promoter elements. Cre recombinase activity is observed specifically in Hb9-expressing interneurons. This strain may be useful in studies of neural circuits.

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Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Mnx1-cre/ERT2)8Rbro show more close
Allele Name	transgene insertion 8 show more close
Allele Attributes	Recombinase-expressing; Inducible show more close
Gene Symbol	Tg(Mnx1-cre/ERT2)8Rbro show more close
Gene Name	transgene insertion 8 show more close
Chromosome	UN show more close
MGI Accession ID	6758656 show more close
Strain of Origin	C57BL/6 x CBA show more close
Molecular Note	The bacterial artificial chromosome (BAC) clone RP24-351I23 containing the mouse Mnx1 (Hb9) gene was modified using recombineering bacterial strain to introduce the Cre/ERT2-BGH polyA and Frt-Zeo-Frt selection cassette into the exon 1 replacing the Mnx1 coding region. The Frt-Zeo-Frt cassette is retained in the transgene but lacks a eukaryotic promoter. Three founder lines (4, 8, and 10) were generated. Line 10 gave low-level inducible activity and was terminated. Line 4 and Line 8 displayed similar inducible recombination activity in the desired cell populations and were maintained. Lines 4 and 8 were indistinguishable, and line 8 was used for the majority of studies described. show more close

HUSBANDRY
Suggested Controls	C57BL/6J show more close
Breeding Considerations	Hemizygotes are viable and fertile. Homozygote viability has not been assessed. show more close

For Research Use Only.