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B6;129S4-Gt(ROSA)26Sortm2(CAG-HIST1H2BB/EGFP)Zjh/J

Cat. No.: CEMM-07250677

Common Name: HG dual
These HG-dual mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette and a frt-flanked STOP cassette preventing transcription of a CAG promoter-driven enhanced green fluorescent protein (EGFP). EGFP is only expressed following Cre-mediated and Flp-mediated recombination. This strain may be useful as an intersectional reporter for cre and FLP expression.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm2(CAG-HIST1H2BB/EGFP)Zjh
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Allele Name
targeted mutation 2
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Allele Attributes
Conditional ready (e.g. floxed); Reporter
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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Expressed Genes
H2B-eGFP, Histone H2B-enhanced Green Fluorescent Protein
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MGI Accession ID
5700497
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Site of Expression
After Cre- and FLP-recombination, EGFP fluorescence is expected in cells/tissues where the expression patterns of the individual promoters driving Cre and Flp overlap.
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Strain of Origin
(C57BL/6 x 129S4/SvJae)F1
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Molecular Note
The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, a FRT flanked STOP cassette, an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by an enhanced green fluorescent protein sequence), and a Neo-polyA cassette. The entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
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HUSBANDRY
Breeding Considerations
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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