Online Inquiry
B6.Cg-Tg(CAG-cre/Esr1*)5Amc/J
Cat. No.: CEMM-07250019
Common Name: CAGGCre-ERTM
When these CAGGCre-ERTM mice are bred with mice carrying a floxed target gene, tamoxifen-inducible Cre-mediated recombination results in deletion of the floxed sequences in widespread cells or tissues of the offspring, including developing embryos and cultured cells derived from transgenic mice.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(CAG-cre/Esr1*)5Amc
show more
close
|
| Allele Name |
transgene insertion 5
show more
close
|
| Allele Attributes |
Recombinase-expressing; Inducible
show more
close
|
| Gene Symbol |
Tg(CAG-cre/Esr1*)5Amc
show more
close
|
| Gene Name |
transgene insertion 5
show more
close
|
| Chromosome |
UN
show more
close
|
| Expressed Genes |
cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
Tamoxifen-inducible cre; widespread pattern of expression; Tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice
show more
close
|
| Strain of Origin |
(C57BL/6 x CBA)F1
show more
close
|
| Molecular Note |
This transgene expresses a fusion protein consisting of Cre recombinase joined to the ligand-binding domain of a mouse estrogen receptor modified to bind to 4-hydroxytamoxifen, but not to endogenous estrogen. The CAG promoter, containing a chicken beta actin promoter/enhancer coupled with the cytomegalovirus immediate-early (CMV-IE) enhancer, drives high levels of expression in most tissues. In the presence of tamoxifen, the fusion protein is transported into the nucleus, where Cre can excise loxP-flanked segments from conditionally modified genes.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
show more
close
|
| Breeding Considerations |
Homozygous mice are not viable or fertile. Hemizygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When maintaining our live colony, hemizygous mice may be bred with wildtype (non-carrier) mice from the colony. The expected coat color is black nonagouti.
show more
close
|
| Breeding Strategy |
C57BL/6J x Hemizygote; Hemizygous x C57BL/6J
show more
close
|
For Research Use Only.
Related Products