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B6.Cg-Spry1tm1.1Ssan Spry2tm1Ssan/J

Cat. No.: CEMM-07250934

Common Name: Spry1/2flx; Spry1/2-floxed [Spry1flx Spry2flx]
Spry1/2flx mice harbor two floxed alleles - Spry1flx and Spry2flx. For each allele, removal of the floxed sequences deletes the protein-coding region - creating a null allele. These mice may be useful in studying T cell immunity (for example, negative regulation of T cell signaling via inhibition of Ras-MAPK signaling, or altering T cell metabolism for enhancing antitumor effects).
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Spry2tm1Ssan
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Allele Name
targeted mutation 1
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Allele Attributes
Conditional ready (e.g. floxed)
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Gene Symbol
Spry2
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Gene Name
sprouty RTK signaling antagonist 2
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Chromosome
14
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MGI Accession ID
6209492
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Strain of Origin
B6.Cg-Thy1a
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Molecular Note
Exon 2 was flanked by a loxP site on one side and a loxP site flanked neomycin resistance gene cassette on the other. This Creates a conditional-ready allele with exon 2 (containing the entire coding region) floxed.
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HUSBANDRY
Suggested Controls
C57BL/6NJ
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Breeding Considerations
Spry1/2flx mice harbor two floxed alleles - Spry1flx on chromosome 3 and Spry2flx on chromosome 14 - each of which will segregate independently. Double homozygous mice are viable and fertile with normal breeding, and no reported gross phenotypic abnormalities. When maintaining our live colony, double heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6N inbred mice. Alternatively, double homozygous mice may be bred together.
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Breeding Strategy
Homozygous Spry1, Homozygous Spry2 x Homozygous Spry1, Homozygous Spry2
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For Research Use Only.
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