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B6(Cg)-Calb2tm2.1(cre/ERT2)Zjh/J
Cat. No.: CEMM-07250280
The Calb2-CreERT2 (CR-CreER or CR-CreERT2) knock-in allele was designed to both abolish Calb2 (calbindin 2; also called calretinin or CR) gene function and direct CreERT2 fusion protein expression to calretinin positive interneurons in the brain by the endogenous Calb2 promoter/enhancer elements. Cre-ERT2 fusion gene activity is inducible by tamoxifen administration.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Calb2tm2.1(cre/ERT2)Zjh
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| Allele Name |
targeted mutation 2.1
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| Allele Attributes |
Recombinase-expressing; Inducible
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| Gene Symbol |
Calb2
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| Gene Name |
calbindin 2
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| Chromosome |
8
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| Expressed Genes |
cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene
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| MGI Accession ID | show more close |
| Site of Expression |
Following Tamoxifen administration, Cre recombinase activity is observed in calretinin positive interneurons in the brain.
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| Strain of Origin |
C57BL/6
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| Molecular Note |
A targeting vector was designed to insert a CreERT2 fusion gene, an SV40 polyA signal, and anFRT flanked neo cassette into the initiation codon of the Calb2 (calbindin 2; also called calretinin or CR) locus. This construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Mutant mice were bred with Actin-FLPe mice to remove the neo cassette,
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J mice. The donating investigator has not characterized the homozygous phenotype to date.
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For Research Use Only.
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