B6.Cg-Penk^{tm1.1(cre/ERT2)Hze}/J

Penk^{tm1.1(cre/ERT2)Hze}

targeted mutation 1.1

Recombinase-expressing; Inducible

Penk

preproenkephalin

4

cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene

5501235

Cre recombinase expression (in situ hybridization) is strong in striatum, in olfactory tubercle, and in sparse dentate gyrus and cortex, and is observed weakly throughout the brain.

(129S6/SvEvTac x C57BL/6NCrl)F1

A targeting vector contained a partial Penk intron 1 sequence containing an frt3 site, a partial Penk exon 2 sequence up to (but not including) the endogenous stop codon, a self-cleaving T2A sequence in-frame with the Nxph4 coding sequence, a Cre/ERT2 fusion gene, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, an AttP site and genomic sequence from the Penk locus starting 509 bp downstream of the endogenous stop codon (to Create a 511 bp deletion including, the stop codon). This targeting vector was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace it with the recombined AttB/AttP site (AttL).

C57BL/6J Wild-type from the colony

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice. The phenotype of homozygous mice has not yet been determined.