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B6;129-Gt(ROSA)26Sortm6(Fzd6)Nat/J
Cat. No.: CEMM-07250517
Common Name: R26-LSL-3xHA-Fz6
This knock-in line at the Gt(ROSA)26Sor (gene trap ROSA 26, Philippe Soriano) locus gives no expression of triple hemagglutinin (HA) epitope-tagged Fzd6 (frizzled homolog 6 (Drosophila)) in the absence of cre-mediated recombination. Following cre-mediated recombination, a loxP-stop-loxP cassette is excised and the downstream triple HA epitope-tagged Fzd6 coding region is expressed, driven by the Gt(ROSA)26Sor promoter.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm6(Fzd6)Nat
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| Allele Name |
targeted mutation 6
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| Allele Attributes |
Conditional ready (e.g. floxed); Inserted expressed sequence; No functional change
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
Fzd6, frizzled class receptor 6, mouse, laboratory; HA tag
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| MGI Accession ID | show more close |
| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
The following elements were inserted, in the following order, into the locus by homologous recombination: loxP-three stop transcription signals-loxP, a Fzd6 coding region with a triple hemagglutinin (HA) tag inserted immediately C-terminal to the signal peptide.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
Homozygotes and heterozygotes are viable and fertile.
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For Research Use Only.
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