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B6.Cg-Igs7tm78.1(tetO-VSFPB1.2)Hze/J
Cat. No.: CEMM-07250505
Common Name: Ai78(TITL-VSFPB1.2)-D; Ai78D
Ai78(TITL-VSFPB)-D (also called Ai78D) mice are a Cre/Tet-dependent, voltage-sensitive, fluorescent line, created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). After removal of the floxed-STOP cassette by Cre recombinase, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of the voltage-sensitive fluorescent protein Butterfly 1.2 (VSFP-Butterfly1.2 or VSFPB1.2). VSFP-Butterfly1.2 exhibits voltage-dependent FRET upon membrane depolarization, and enables optical recordings at high spatial resolution with intrinsic assignment of recorded signals to VSFP-targeted cell populations (such as layer 2/3 pyramidal cells).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm78.1(tetO-VSFPB1.2)Hze
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| Allele Name |
targeted mutation 78.1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inducible
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| Gene Symbol |
Igs7
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| Gene Name |
intergenic site 7
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| Chromosome |
9
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| Expressed Genes |
VSFPB1.2, voltage-sensitive fluorescent protein Butterfly 1.2,
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| MGI Accession ID | show more close |
| Site of Expression |
Expression of VSFPB1.2 is under the control of tetO in cells/tissues where promoters driving Cre recombinase are expressed.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The targeting vector was designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a modified Tet response element (TRE or tetO), a floxed-STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), the voltage-sensitive fluorescent protein Butterfly 1.2 coding sequence (details below), a WPRE sequence (to enhance mRNA stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, an AttB site, a PGK-5 hygro cassette, an RNA spice donor and a FRT5 site. Embryonic stem cells, previously targeted with FRT5-AttB-Hygro2 cassette-AttP-SV40pA-splice acceptor-PGKpA-FRT3 in the same locus, were re-transfected with the targeting vector and Flp recombinase vector. Mutant mice were bred with Gt(ROSA)26Sortm3(phiC31*)Sor mice to remove to remove the AttB/AttP-flanked PGK-hygro-SV40pA cassette and replace it with the recombined AttB/AttP site (AttL). The Ai78D allele is therefore TIGRE-frt3-Ins-TRE-LSL-VSFPB1.2-WPRE-bGHpA-Ins-AttL.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice. The donating investigator has not attempted to generate homozygous mice to date.
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For Research Use Only.
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