Online Inquiry
B6.Cg-Gt(ROSA)26Sortm4(Ikbkb)Rsky/J
Cat. No.: CEMM-07250100
Common Name: R26-STOPFLikk2ca
These R26StopFLikk2ca mice allow inducible expression of an activated form of Ikbkb (IKK2ca) and subsequent activation of the NF-kappaB transcription factor pathways.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm4(Ikbkb)Rsky
show more
close
|
| Allele Name |
targeted mutation 4
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Constitutively active; Inserted expressed sequence
show more
close
|
| Gene Symbol |
Gt(ROSA)26Sor
show more
close
|
| Gene Name |
gene trap ROSA 26
show more
close
|
| Chromosome |
6
show more
close
|
| Expressed Genes |
Ikbkb, inhibitor of kappaB kinase beta, mouse, laboratory; GFP, Green Fluorescent Protein
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
Expression of the bicistronic sequences (a constitutively active form of IKbkb (IKK2ca) and EGFP) is blocked by a loxP-flanked STOP cassette. When bred to Cre recombinase expressing mice, the loxP-flanked STOP cassette is removed, resulting in expression of the IKK2ca and EGFP in Cre recombinase expressing cells.
show more
close
|
| Strain of Origin |
B6.Cg-Thy1a
show more
close
|
| Molecular Note |
An IRES-eGFP cassette and a cDNA sequence encoding Ikbkb containing two serine to glutamate substitutions in the activation loop of the kinase domain, preceded by a floxed stop-neo cassette was inserted into the locus. The Ikbkb would be expressed under the control of the endogenous ROSA26 promoter after Cre-mediated excision of the STOP cassette.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
show more
close
|
| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred.
show more
close
|
| Breeding Strategy |
Homozygote x Homozygote
show more
close
|
For Research Use Only.
Related Products