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STOCK Gt(ROSA)26Sortm10.1(CAG-birA*, -mKate2)Amc/J

Cat. No.: CEMM-07251111

Common Name: R26 BirA*G3-ER
R26 BirA*G3 knock-in mice have a CAG promoter controlling the Cre-inducible expression of a myc-tagged endoplasmic reticulum-directed promiscuous biotin ligase sequence, BirA*G3-ER. Cre-mediated recombination results in the excision of a floxed GFP sequence, enabling the tissue and time-dependent expression of BirA*G3 and mKate2. This is a tool for the isolation and purification of proteins and protein complexes trafficking through the secretory pathway upon biotin administration.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm10.1(CAG-birA*,-mKate2)Amc
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Allele Name
targeted mutation 10.1
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Allele Attributes
Conditional ready (e.g. floxed); Reporter
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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MGI Accession ID
7279067
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Strain of Origin
B6(Cg)-Tyrc-2J/J
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Molecular Note
The targeting vector is designed with, from 5' to 3', a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a floxed cassette containing a green fluorescent protein (GFP) sequence and a polyadenylation signal, a myc-tagged endoplasmic reticulum-directed promiscuous biotin ligase sequence (BirA*G3-ER), an internal ribosome entry site (IRES), and an mKate2 red fluorescent protein sequence. This targeting vector, which also includes an attB-neoR-attP cassette, was inserted into the Gt(ROSA)26Sor gene. PhiC31o-mediated recombination removed the neo cassette.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony, homozygous mice may be bred together.
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Breeding Strategy
Heterozygote x Heterozygote
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For Research Use Only.
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