B6.Cg-Ccn2^{tm1.1(folA/cre)Hze}/J

Ccn2^{tm1.1(folA/cre)Hze}

targeted mutation 1.1

Recombinase-expressing; Inducible

Ccn2

cellular communication network factor 1

10

Ccn2, cellular communication network factor 1, mouse, laboratory; GFP/Cre, Green Fluorescent Protein/Cre recombinase fusion protein

5702703

When induced, Cre recombinase activity is restricted to layer 6b of the cortex and in restricted populations within the cortical subplate of the brain.

(129S6/SvEvTac x C57BL/6NCrl)F1

The targeting vector contains, from 5' to 3', a partial exon 1 through intron 4 sequence containing an FRT3 site in intron 4, a partial exon 5 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the coding sequence, a dgCre fusion gene (described below) that is in-frame with the coding sequence, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an FRT5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. The dgCre fusion gene is an enhanced green fluorescent protein/Cre recombinase fusion gene with an N terminal fusion of the first 159 amino acids of the Escherichia coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to also include the R12Y/Y100I destabilizing domain mutations. PhiC31 integrase-mediated recombination removed the AttB/AttP-flanked sequences. Trimethoprim-treatment is required to stabilize the Cre protein. No EGFP is detected with or without induction.

C57BL/6J

When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. it has not been attempted to make this strain homozygous.

Wild-type x Heterozygote; Heterozygote x Wild-type