B6.Cg-Tg(Avil-icre/ERT2)AJwo/J

Tg(Avil-icre/ERT2)AJwo

transgene insertion A

Recombinase-expressing; Inducible

Tg(Avil-icre/ERT2)AJwo

transgene insertion A

6

cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene

5312922

When induced by Tamoxifen, Cre recombinase activity is observed in dorsal root ganglia and sensory neurons.

Not Specified

BAC recombineering was used to generate a vector with the Cre/ERT2 sequence fused with the start codon in exon 2 of a partial advillin gene sequence located in BAC clone RPCI23-424F19. An Frt-flanked kanamycin selection marker was included in the initial Cre cassette and was subsequently excised by induction of Flp recombinase in EL250 cells. The purified construct was used for pronuclear injection to generate 8 founder lines. Cre activity was verified in offspring of founders by breeding to ROSA26-lacZ reporter mice. Founder A exhibited strongest staining in DRGs and was analyzed in detail. Line A carries about 10 transgene copies (estimated by Southern blotting.) The transgene has integrated into the intronic region between exons 10 and 11 of the Ccser1 gene on chromosome 6 (chr6: 62276099-62638573). The insertion Creates an 82 bp deletion.

C57BL/6J

When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings or to C57BL/6J inbred mice. Mice that are hemizygous for the transgene are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous to date. As of January 2017, it has been the experience Repository that when breeding hemizygous mice to wildtype siblings or C57BL/6J inbred mice, the resulting litters have less hemizygous offspring than expected, which is 29% instead of the expected 50%. Additionally in those matings, we observe significant incidence of hemizygous males with tail damage and/or genital injury.See strain description (phenotype) for important information on assessing transgene copy number each generation.

Noncarrier x Hemizygote; Hemizygote x Noncarrier