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B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J

Cat. No.: CEMM-07250051

These ZW mice harbor a Cre-conditional pCZW transgene and have widespread expression of lacZ under the control of the CMV enhancer/chicken actin promoter. When bred to mice expressing Cre recombinase, the "floxed" lacZ sequence is removed in the resulting offspring, and lacZ expression is replaced by wheat germ agglutinin (plant lectin) expression in all cre-expressing cells. These ZW mice may be useful for global expression of lacZ or, when crossed to a cre-expressing strain, for tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the spinal cord.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Tg(CAG-lacZ-WGA)330Bbm
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Allele Name
transgene insertion 330
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Allele Attributes
Reporter
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Gene Symbol
Tg(CAG-lacZ-WGA)330Bbm
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Gene Name
transgene insertion 330
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Chromosome
UN
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Expressed Genes
WGA, Wheat Germ Agglutin, truncated; lacZ, beta-galactosidase, E. coli
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MGI Accession ID
3052324
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Site of Expression
LacZ is expressed throughout central and peripheral nervous system; When crossed with a Cre recombinase-expressing strain, LacZ expression is replaced with wheat germ agglutinin expression
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Strain of Origin
(C57BL/6J x CBA/J)F1
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Molecular Note
The transgene is comprised of a minimal immediate-early CMV enhancer/chicken beta-actin promoter driving ubiquitous expression of a lox-P flanked beta-galactosidase/neomycin phosphotransferase II (beta-geo) fusion gene that terminates in a strong, triple polyadenylation signal, followed by a C-terminally truncated wheat germ agglutinin coding sequence. Neuron-specific excision of the beta-geo gene by Cre recombinase permits expression of the lectin, whose migration allows tracing of synapses involving the expressing cells.
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HUSBANDRY
Suggested Controls
B6CBAF1/J Noncarrier
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Breeding Considerations
When maintaining a live colony, hemizygous mice are bred to wildtype siblings or to B6CBAF1/J. It is reported that some animals genotyping positive for the transgene may not express the transgene; it is strongly recommended to also test animals by lacZ staining of ear notches.
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For Research Use Only.
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