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B6.129S6-Tg(CAG-Bgeo, -SMN2)E9Dscd/J
Cat. No.: CEMM-07250400
These Tg hSMN2 E9 inducible SMN transgenic mice conditionally express a human SMN2 cDNA. Widespread lacZ expression is observed except in those tissues where cre recombination has resulted in the human SMN2 cDNA to be expressed instead. These transgenic mice are suitable for use in studies related to Spinal Muscular Atrophy.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(CAG-Bgeo,-SMN2)E9Dscd
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| Allele Name |
transgene insertion E9
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence; Humanized sequence
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| Gene Symbol |
Tg(CAG-Bgeo,-SMN2)E9Dscd
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| Gene Name |
transgene insertion E9
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| Chromosome |
UN
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| Expressed Genes |
Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli; SMN2, survival of motor neuron 2, centromeric, human
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| MGI Accession ID | show more close |
| Site of Expression |
LacZ expression is seen in all cells prior to Cre recombination. After Cre excision of the floxed- LacZ-neo cassette, human SMN2 is expressed in Cre-expressing tissues.
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| Strain of Origin |
129S6/SvEvTac
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| Molecular Note |
The pCCALL2/hSMN2 transgene was designed with, from 5' to 3', the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (originally from the pCAGG vector), a loxP-flanked STOP cassette (beta-galactosidase/neomycin resistance cassette [beta-geo] with three copies of the SV40 polyA signal), the 1.37 kbp full-length human SMN2 cDNA sequence including all exons (1-8) from the start codon to the stop codon, and a rabbit polyA signal. The 1.37 kbp human SMN2 cDNA sequence (encoding exons 1-8) was originally derived via RT-PCR performed on total RNA isolated from SMA type 1-derived patient fibroblast cells (Coriell Institute GM 3813; shown to express both full-length and truncated SMN2 transcripts). Lines A9 and E9 was generated.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, transgenic mice may be bred with wildtype (non-carrier) mice from the colony or with C57BL/6J inbred mice.
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For Research Use Only.
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