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B6;129S6-Igs7tm2(tetO-Optopatch2)Acoh/J
Cat. No.: CEMM-07250748
Common Name: Optopatch2 Ai141
Conditional expression of Optopatch2 (an optogenetic actuator/indicator) is under the control of a tetracycline response element (TRE; tetO) and floxed Stop integrated in the Igs7 (TIGRE) locus. Functionality of Optopatch2 requires the expression of tTA as well as cre recombinase-mediated excision of the floxed Stop. This is line Ai141.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm2(tetO-Optopatch2)Acoh
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inducible
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| Gene Symbol |
Igs7
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| Gene Name |
intergenic site 7
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| Chromosome |
9
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| Expressed Genes |
RFP, Red Fluorescent Protein, coral; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Optopatch2 functionality is achieved in Cre expressing tissues after tetracycline-controlled transactivator (tTA) and Cre recombinase expression.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The targeting construct contains a tetracycline response element (TRE;tetO)-loxP-Stop-loxP-Optopatch2. Optopatch2 is a bicistronic construct that co-expresses QuasAr2-dark mOrange2 (an enhanced archaerhodopsin-3 (Arch)-based voltage indicator) and CheRiff-EGFP (a sensitive blue-shifted optogenetic actuator) separated by the porcine teschovirus-1 (P2A) peptide.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
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| Breeding Considerations |
Heterozygotes and homozygotes are viable and fertile.
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For Research Use Only.
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