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STOCK Gt(ROSA)26Sortm2(CAG-EYFP*, -mCherry*, -EGFP*, -mCerulean*)Ben/J

Cat. No.: CEMM-07250855

Common Name: iChr2-Control-Mosaic
iChr2-Control-Mosaic mice allow three possible Cre recombination outcomes in any single cell. Each outcome results in expression of a chromatin/nuclear-targeted fluorescent protein, and each is mutually exclusive and permanent. These mice are also designed to permit further introduction of genes of interest for inducible, fluorescent and functional genetic mosaic analysis (ifgMosaic). Mice are available with the similarly-designed iMb2-Control-Mosaic allele harboring membrane-bound fluorescent proteins. In addition, the iMb2-Control-Mosaic::iChr2-Control-Mosaic compound heterozygous mice harbor both alleles.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm2(CAG-EYFP*,-mCherry*,-EGFP*,-mCerulean*)Ben
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Allele Name
targeted mutation 2
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Allele Attributes
Conditional ready (e.g. floxed); Reporter; Epitope tag
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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Expressed Genes
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral; YFP, Yellow Fluorescent Protein, jellyfish; CFP, Cyan Fluorescent Protein, jellyfish; HA tag
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MGI Accession ID
6108166
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Site of Expression
Fluorescent proteins will be expressed in in tissues expressing Cre recombinase driven by a selected promoter.
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Strain of Origin
(129S6/SvEvTac x C57BL/6NCrl)F1
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Molecular Note
This allele has three chromatin/nuclear-targeted fluorescent protein sequences uniquely flanked with pairs of incompatible lox sites (in the same direction), all inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The targeting vector is designed with (from 5' to 3') two copies of chicken beta-globin HS4 insulator element (2xINS; inCrease downstream gene expression and minimize regulatory interference), a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxN site, a lox2272 site, a loxP site, the N-PhiMut gene (non-cytotoxic/non-fluorescent Phialidium yellow fluorescent protein variant PhiYFP with nuclear localization signal), SV40 polyA signal sequence, a second loxN site, a full consensus KOZAK sequence, the 6xHis-H2B-mCherry gene (monomeric DsRed fluorescent protein variant mCherry with N-terminal 6xHis affinity tag and 125 amino acid human histone 2B chromatin/nucleus tag), in-frame Thosea asigna virus 2A cleavage site (T2A; mediates ribosomal skipping), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance mRNA transcript stability), SV40 polyA signal sequence, a second lox2272 site, a full consensus KOZAK sequence, the H2B-EGFP gene (enhanced green fluorescent protein with 127 amino acid human histone 2B chromatin/nucleus tag and KOZAK sequence plus C-terminal paramyxovirus V5 tag sequence), in-frame T2A sequence, WPRE sequence, SV40 polyA signal sequence, a second loxP site, a full consensus KOZAK sequence, the HA-H2B-mCerulean gene (monomeric enhanced cyan fluorescent protein variant mCerulean with N-terminal hemagglutinin (HA) tag, 125 amino acid human histone 2B chromatin/nucleus tag and KOZAK sequence), in-frame T2A sequence, WPRE sequence, SV40 polyA signal sequence, an FRT site flanked neomycin resistance gene cassette, and a second 2xINS sequence.
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HUSBANDRY
Suggested Controls
B6129SF2/J
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Breeding Considerations
Before exposure to Cre recombianse, the donating investigator reports that homozygous mice are viable and fertile with normal breeding, and exhibit no behavioral, neurological or any other spontaneous abnormalities. When maintaining a live colony, homozygous mice may be bred together.
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Breeding Strategy
Homozygote x Homozygote
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For Research Use Only.
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