B6(129S4)-Et(icre/ERT2)10596Rdav/J

Cat. No.: CEMM-07250196

Common Name: C57BL/6-Et(icre/ERT2)10596Rdav/J

These mice express the iCre-ERT2 fusion protein (iCre-ERT2) under the control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. These mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in brain tissues.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Et(icre/ERT2)10596Rdav show more close
Allele Name	enhancer trap 10596 show more close
Allele Attributes	Recombinase-expressing; Inducible show more close
Gene Symbol	Et(icre/ERT2)10596Rdav show more close
Gene Name	enhancer trap 10596 show more close
Chromosome	UN show more close
Expressed Genes	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene show more close
MGI Accession ID	3852351 show more close
Site of Expression	Cre recombinase activity is observed in brain tissues following Tamoxifen administration (although some Cre activity is reported prior to Tamoxifen administration). show more close
Strain of Origin	C57BL/6J show more close
Molecular Note	An enhancer trap transgenic approach was used to generate these mice. The hsyn-bgi-Cre:ERT2-bGHpA transgene was designed with the human synapsin minimal promoter (hsyn) minimal promoter driving an iCre-ERT2 fusion gene (a codon-improved Cre recombinase sequence fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). A beta globin intron separates the promoter from the Cre gene and the bovine growth hormone polyadenylation signal follows the Cre sequence. This transgene was injected into C57BL/6J embryos. Founder line was established on a C57BL/6J genetic background. show more close

HUSBANDRY
Suggested Controls	C57BL/6J Noncarrier show more close
Breeding Considerations	When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (non-carrier) siblings, or to C57BL/6J inbred mice. show more close

For Research Use Only.