B6(129S4)-Et(cre/ERT2)13866Rdav/J

Cat. No.: CEMM-07250236

These mice express Cre-ERT2 fusion protein (Cre-ERT2) under the control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. These mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in brain tissues.

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Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Et(cre/ERT2)13866Rdav show more close
Allele Name	enhancer trap 13866 show more close
Allele Attributes	Recombinase-expressing; Inducible show more close
Gene Symbol	Et(cre/ERT2)13866Rdav show more close
Gene Name	enhancer trap 13866 show more close
Chromosome	UN show more close
Expressed Genes	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene show more close
MGI Accession ID	4947562 show more close
Site of Expression	Cre recombinase activity is found in amygdala, ventral striatum, hypothalamus, pallidum, and midbrain tissue of the brain. show more close
Strain of Origin	C57BL/6J show more close
Molecular Note	An enhancer trap lentiviral transgenic approach was used to generate these mice. The transgene was designed with a self-inactivating lentiviral vector backbone (FUGW), a human synapsin minimal promoter (-244 to +47), a beta-globin intron, a Cre/ERT2 fusion gene (Cre fused to a mutated human estrogen receptor ligand binding domain), and a bovine growth hormone polyA signal, all antisense orientation relative to the lentiviral transcript. This packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J mouse embryos. Founders were crossed to C57BL/6 animals. Animals were crossed to B6-Gt(ROSA)26Sortm1Sor mice to assess Cre activity/transmission. Cre positive, lacZ negative mice were subsequently bred to C57BL/6 mice to Create this line. show more close

HUSBANDRY
Suggested Controls	C57BL/6J Noncarrier show more close
Breeding Considerations	When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (non-carrier) siblings, or to C57BL/6J inbred mice. show more close

For Research Use Only.