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B6;129-Syt11tm1Sud/J
Cat. No.: CEMM-07250101
Common Name: Syt11 knock-in; 11E
Exon 2 of the mouse Syt11 (synaptotagmin XI) gene carries a Flag tag insertion and is flanked by loxP sites in this Syt11 knock-in allele. Cre recombinase-mediated excision of the floxed exon results in a knock-out of the gene, useful in studies of neurotransmitter release mechanisms.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Syt11tm1Sud
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
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| Gene Symbol |
Syt11
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| Gene Name |
synaptotagmin XI
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| Chromosome |
3
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| MGI Accession ID | show more close |
| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
A targeting vector was designed to place a LoxP-Flp-neomycin-Flp cassette in intron 1, a FLAG-(C)4-FLAG cassette in exon 2, and LoxP site in intron 2. Mutant protein expression levels are similar to those of the wildtype protein. Expression is brain-specific and localized in the neuronal soma and dendrites (presumably on cargo vesicles). Protein can be recognized using FLAG antibody by Western blot analysis, but not in brain sections and labeled cultures. No significant N-ethyl-maleimide-sensitive factor attachment protein receptor (SNARE) binding is observed in immunoprecipitation (IP) assays.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
Heterozygotes and homozygotes are viable and fertile.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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