B6.Cg-Gt(ROSA)26Sor^{tm1(rtTA, EGFP)Nagy}/J

Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}

targeted mutation 1

Conditional ready (e.g. floxed); Reporter; Transactivator

Gt(ROSA)26Sor

gene trap ROSA 26

6

rtTA, reverse tetracycline-controlled transactivator, E. coli; GFP, Green Fluorescent Protein

3583817

When these mutant mice are crossed to a strain expressing Cre recombinase, rtTA and EGFP are expressed in the Cre-expressing tissues. These mice can be used to generate mutant mice in which the tissue specificity of Cre-expression and doxycycline inducibility of the rtTA can be combined.

(129X1/SvJ x 129S1/Sv)F1-Kitl+

A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Upon Cre expression, the floxed neo cassette is removed and the ROSA26 promoter drives expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene.

C57BL/6J

Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred.

Homozygote x Homozygote