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B6;129-Gt(ROSA)26Sortm1Sho/J
Cat. No.: CEMM-07250008
The β-galactosidase-neomycin phosphotransferase fusion gene (βgeo)-trapped reverse orientation splice acceptor βgeo line 26 (ROSA26) locus was modified by gene targeting such that βgeo is expressed only after Cre-mediated excision of loxP-flanked DNA sequences. These mice may prove useful in the study of cell fate and cell migration during embryogenesis through recombinase-activated tagging.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1Sho
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli
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| MGI Accession ID | show more close |
| Site of Expression |
When crossed to a Cre recombinase-expressing strain, LacZ expression is observed in the Cre-expressing tissues; this is similar to Gt(ROSA)26Sortm1Sor but has one copy of the loxP-flanked reporter gene
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| Strain of Origin |
129S4/SvJaeSor
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| Molecular Note |
A targeting vector was designed to insert floxed (flanked by loxP) phosphoglycerate kinase-1-cytosine deaminase/phosphoglycerate kinase-1-puromycin (PGK-CD/PGK-Puro) and stop sequences between the splicing acceptor (SA) and the beta-gal-neomycin phosphotransferase fusion gene (beta-geo) sequences of the proviral ROSA 26 locus. Correctly targeted, this resulted in ES cells that were Puro resistant but G418 susceptible, and did not express beta-gal.
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For Research Use Only.
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