CBy.B6-Gt(ROSA)26Sor^{tm1(HBEGF)Awai}/J

Gt(ROSA)26Sor^{tm1(HBEGF)Awai}

targeted mutation 1

Conditional ready (e.g. floxed); Inserted expressed sequence

Gt(ROSA)26Sor

gene trap ROSA 26

6

HBEGF, heparin binding EGF like growth factor, chimpanzee; DTR, Simian Diphtheria Toxin Receptor

3772576

B6.Cg-Thy1a

A targeting vector was designed with a loxP-flanked STOP cassette upstream of the open reading frame of the simian Diphtheria Toxin Receptor (DTR; from simian hbEGF cDNA (base pair 56-682)). The loxP-flanked region contains two SV40 polyA signals, an FRT flanked neomycin resistance gene, and a transcriptional STOP cassette. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived Bruce4 ES embryonic stem (ES) cells. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.

BALB/cByJ

Mutant mice were bred to BALB/cByJ mice to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.