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STOCK Fostm2.1(icre/ERT2)Luo/J
Cat. No.: CEMM-07250793
Common Name: Fos2A-iCreER (TRAP2)
Fos2A-iCreER knock-in (also called Fos2A-iCreERT2 or "TRAP2") was designed to have expression of a tamoxifen-inducible, improved Cre recombinase (icre/ERT2) from the Fos promoter/enhancer elements - without disrupting endogenous Fos expression. Fos2A-iCreER mice are a Cre-lox tool allowing inducible iCre recombination in Fos-expressing cells/tissues (including neurons activated by specific somatosensory, visual and auditory stimuli) or behavioral experiences such as enriched environment, fear conditioning or water deprivation. Immediate early genes (IEGs) are the connection between gene expression and a neuron's electrical/synaptic activity (which defines neuronal response properties).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Fostm2.1(icre/ERT2)Luo
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| Allele Name |
targeted mutation 2.1
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| Allele Attributes |
Recombinase-expressing; Inducible
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| Gene Symbol |
Fos
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| Gene Name |
FBJ osteosarcoma oncogene
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| Chromosome |
12
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| Expressed Genes |
cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene
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| MGI Accession ID | show more close |
| Site of Expression |
When these mice are bred with mice containing lox-flanked sequences, Tamoxifen-inducible iCre-mediated recombination will result in deletion of floxed sequences in the Fos-expressing cells of the offspring.
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| Strain of Origin |
129
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| Molecular Note |
A targeting vector was designed to insert a 2A::iCreER::FRT neo-FRT cassette into the translational stop site of the FBJ osteosarcoma oncogene locus (Fos) on chromosome 12. The viral 2A oligopeptide sequence mediates ribosomal skipping. The iCreER fusion gene used here has an improved/optimized variant of Cre recombinase (iCre) that is fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain (ER). TThe targeting construct was electroporated into 129;FVB-derived embryonic stem (ES) cells, and correctly targeted ES cells were injected into recipient blastocysts. Chimeric animals were bred to CD1 mice and/or germline-active GFP-FlpO transgenic mice (mixed genetic background including CD1) to delete the FRT flanked neo cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6NJ inbred mice. Alternatively, homozygous mice are viable and fertile, and may be bred together. CD BioScience live colony observed coat colors are black or white (albino)
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| Breeding Strategy |
Homozygote x Homozygote
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| Coat Color |
Coat colors are black or white (albino).
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For Research Use Only.
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