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B6.129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J
Cat. No.: CEMM-07250036
Common Name: MADM-GG
These knock-in mice were developed to serve as controls for use with the mosaic analysis with double markers (MADM) system that allows simultaneous labeling and gene knockout in clones of somatic cells or isolated single cells in vivo. This strain carries only the green fluorescent protein, EGFP (G).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-EGFP)Luo
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Widespread expression
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
A construct containing the CMV beta-actin enhancer/promoter, SV40 poly(A) signal, and encoding the N-terminal and C-terminal halves of a mutant enhanced green fluorescent protein (EGFP) separated by a beta-globin intron containing a loxP-flanked neomycin resistance gene, was knocked into ES cells at the ROSA26 locus. The two parts of the coding sequence are separated by the intron but still in the same reading frame. Ubiquitous GFP expression occurs in the absence of Cre-mediated recombination.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J Wild-type from the colony
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| Breeding Considerations |
When maintaining a live colony, these mice are bred as heterozygotes. The fertility of homozygous mutants on the C57BL/6 genetic background currently is under assessment.
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For Research Use Only.
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