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B6;129-Gt(ROSA)26Sortm1(CAG-COP4*E123T*H134R, -tdTomato)Gfng/J
Cat. No.: CEMM-07250337
Common Name: R26-CAG-LSL-2XChETA-tdTomato
The R26-CAG-LSL-2XChETA-tdTomato reporter allele is designed to express two individual proteins following exposure to Cre recombinase; the ChR2-E123T accelerated (ChETA)/H134R variant of channelrhodopsin-2 and tdTomato fluorescent protein gene. When used with neuron-specific Cre recombinase expressing strains, the double mutant mice can be used in optogenetic studies and visualization of whole neurons.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-COP4*E123T*H134R,-tdTomato)Gfng
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
COP4, Channelrhodopsin, Chlamydomonas
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| MGI Accession ID | show more close |
| Site of Expression |
Cre excision of the stop signal results in expression of a channelrhodopsin-2 (COP4) and tdTomato in Cre-expressing tissues.
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
A targeting vector, which contained the CAG promoter, a loxP site flanked STOP fragment and pGK-NEO-pA cassette, 2 copies of channelrhodopsin-2 with E123T mutation (ChETA variant) and the H134R mutation, P2A-tdTomato variant of Enhanced Red Fluorescent Protein, and WPRE (Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element) sequence to enhance expression level, was inserted into the locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
When maintaining a live colony, the Donating Investigator reports that these mice can be bred as homozygotes.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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