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B6.129-Gt(ROSA)26Sortm1(CAG-cas9*, -EGFP)Fezh Is(14)2Rdf/CjwuJ

Cat. No.: CEMM-07251092

Common Name: MDRCas9
MDRCas9 double mutant mice contain the 14qC3-MDR allele, containing a loxP- and frt-flanked region on Chr 14 that has been implicated in B cell chronic lymphocytic leukemia (CLL). They also contain a Rosa26-LSL-Cas9 knock-in allele with cre recombinase-dependent expression of CRISPR associated protein 9 (cas9) endonuclease and EGFP directed by a CAG promoter. When bred to MDRCD19 double homozygous mice, these mice have applications in studies related to tumorigenesis and therapeutic management of chronic lymphocytic leukemia.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Is(14)2Rdf
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Allele Name
insertion
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Allele Attributes
Conditional ready (e.g. floxed); No functional change
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Gene Symbol
Is(14)2Rdf
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Gene Name
insertion
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Chromosome
14
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MGI Accession ID
4437013
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Site of Expression
Cre recombination results in EGFP expression in Cre-expressing tissues.
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Strain of Origin
129S1/Sv-Oca2+ Tyr+ Kitl+
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Molecular Note
LoxP and FRT sites as well as a PGK promoter and Hygro cassette were place about 5 kb telomeric to the alternative exon 1 of Dleu2 by homologous recombination. The targeting was done in ES cells carrying Is(14)1Rdf. The two constructs are in cis configuration.
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HUSBANDRY
Suggested Controls
B6.129S1
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Breeding Considerations
When maintaining a live colony, double homozygous mice may be bred together.
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Breeding Strategy
Heterozygous for Gt(ROSA)26Sor Heterozygous for Is(14)2Rdf x Heterozygous for Gt(ROSA)26Sor Heterozygous for Is(14)2Rdf
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For Research Use Only.
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