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Temporal-specific KO Service
A temporal-specific conditional knockout (cKO) model is a genetically engineered model that allows for the temporal control of gene inactivation in a tissue- or cell-specific manner. In this model, the gene of interest is floxed, meaning that it is flanked by loxP sites, which are specific DNA sequences that allow for site-specific recombination. Creative Biogene offers Temporal-specific KO services based on site-specific recombinase system, which has a wide range of application, including gene functional study, disease mechanism study, drug target validation, and others.
Introduction into Temporal-specific KO
Temporal-specific conditional knockout (cKO) models can be created in various organisms, including zebrafish, fruit flies (Drosophila), and rats, among others. The principle of a Temporal-specific cKO model remains the same across species. The gene of interest is floxed, and an inducible Cre recombinase system is used to activate or inactivate the gene at a specific time point.
Temporal-specific cKO models have broad applications in various fields, including developmental biology, cancer biology, neurobiology, and many others. By controlling the timing of gene inactivation, researchers can gain insights into the role of specific genes in tissue development and disease progression, and potentially identify critical windows of gene function that could be targeted for therapeutic interventions.
Examples of Temporal-specific KO
Temporal-specific KO Mouse
The most popular Temporal-specific KO model is mouse model. By controlling the timing of the knockout, researchers can study the role of a gene at different stages of the mouse's life and in response to different environmental factors or treatments. For example, they can study the effects of knocking out a gene at a specific developmental stage, or in response to a particular disease or drug treatment.
Temporal-specific KO Zebrafish
For example, in zebrafish, CRISPR/Cas9 technology can be used to create a Temporal-specific cKO model. The gene of interest can be flanked by loxP sites, and an inducible Cre recombinase can be introduced into the zebrafish genome using a transgenic approach. When the inducer is administered at a specific time point, the Cre recombinase will excise the gene of interest, leading to gene inactivation in a tissue-specific manner.
Temporal-specific KO Drosophila
Similarly, in Drosophila, the GAL4/UAS system can be used to create a Temporal-specific cKO model. The gene of interest is flanked by loxP sites, and a transgenic fly expressing the GAL4 transcription factor in a tissue-specific or inducible manner is crossed with a fly expressing a UAS-controlled Cre recombinase. When the inducer is administered at a specific time point, the GAL4 transcription factor will activate the UAS-controlled Cre recombinase, leading to the excision of the gene of interest in a tissue-specific manner.
Our Service
Creative Biogene offers Temporal-specific KO service based on site-specific recombinase. We provide Temporal-specific KO service in species included but not limited as follows.
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One-stop Service
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Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
References
- Laurens J. Lambert, et al. (2017). Chapter 15 - Basic Mouse Methods for Clinician Researchers: Harnessing the Mouse for Biomedical Research, Pages 291-312.
- Willemsen, R., et al. (2011). Zebrafish (Danio rerio) as a Model Organism for Dementia. In: De Deyn, P., Van Dam, D. (eds) Animal Models of Dementia. Neuromethods, vol 48. Humana Press.