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Conditional Knockin (KI) Service
Conditional knockin, also refers to inducible KI, is a molecular biology technique used to genetically modify an organism in which a gene of interest is introduced and integrated into the genome of the organism. The key feature of this technique is the ability to control the timing and level of expression of the introduced gene. Creative Biogene offers conditional knockin service in multiple organisms including animals, plants and microbes, as well as in cell lines.
Introduction into Mouse Models Generated by T-SSR System
Conditional knockin (KI) is a genetic engineering technique that allows researchers to control the expression of a particular gene by inserting a piece of DNA containing the gene of interest in the genome of an organism. The gene of interest is placed under the control of an inducible promoter, which can be turn ed on or off using an external stimulus, such as a drug or a chemical compound. Followings are strategies commonly used for generating inducible KI models.
lox-STOP-lox System
The "lox-STOP-lox" system works by placing a "STOP" sequence, which prevents transcription of the downstream gene, between two "loxP" sites. When Cre recombinase is present, it recognizes and binds to the "loxP" sites and catalyzes a recombination event that removes the "STOP" sequence. This allows the downstream gene to be transcribed and translated, resulting in the expression of the gene.
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Flp-FRT Recombination
The Flp-FRT system is similar to the Cre-loxP system but uses a different recombinase, Flp, and its target sites, FRT sites. Like Cre-loxP, the Flp-FRT system can be used to conditionally activate or deactivate gene expression by controlling the location and timing of gene insertion or substitution.
Flip-excision
In this technology, two site-specific recombinase systems are introduced to the genome. When a DNA sequence is flanked by lox sites in opposite directions, Cre reverses the sequence between the sites. If there are lox sites on both sides of the DNA sequence with the same orientation, Cre will excise the sequence. By combining the type, number and orientation of lox sites around the gene of interest, a powerful Flip-excision tool can be created for your specific scientific needs.
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Applications of Conditional Knockin
Conditional gene knockin (cKI) is a genetic engineering technique used to introduce a new gene into a specific cell type or tissue at a specific time. This method has several applications, including:
- Disease modeling: cKI can be used to introduce disease-causing mutations into specific cell types or tissues, allowing researchers to study the effects of these mutations in a more controlled setting.
- Gene therapy: cKI can be used to introduce therapeutic genes into specific cells or tissues, offering a potential treatment for genetic disorders.
- Developmental biology: cKI can be used to study the role of specific genes during embryonic development by introducing the gene of interest into specific cells at specific stages of development.
- Cell lineage tracing: cKI can be used to label specific cells or cell populations with a reporter gene, allowing researchers to track their development and lineage over time.
- Functional genomics: cKI can be used to introduce mutations into specific genes to study their function, allowing researchers to gain insights into gene function in vivo.
Our Service
Creative Biogene offers conditional knockin service in animals included but not limited as follows:
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.