STOCK Tg(Cartpt-cre)1Aibs/J

Cat. No.: CEMM-07250174

Common Name: Cart-Tg1-Cre

These Cart-Tg1-Cre mice express Cre recombinase under the control of the Cartpt (CART prepropeptide) promoter/enhancer regions, and may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, hippocampus, and cerebellum).

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Cartpt-cre)1Aibs show more close
Allele Name	transgene insertion 1 show more close
Allele Attributes	Recombinase-expressing show more close
Gene Symbol	Tg(Cartpt-cre)1Aibs show more close
Gene Name	transgene insertion 1 show more close
Chromosome	UN show more close
Expressed Genes	Cre, Cre recombinase, bacteriophage P1 show more close
MGI Accession ID	3850194 show more close
Site of Expression	Hemizygous Cart-Tg1-Cre mice are viable and fertile, with Cre expression directed to cortex, hippocampus, and cerebellum by the Cartpt promoter/enhancer regions within the BAC transgene. show more close
Strain of Origin	(C57BL/6 x C3H)F1 x C57BL/6 show more close
Molecular Note	The 195 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-379C17, containing the entire Cartpt locus (and other genes), was modified by targeting a Cre recombinase coding sequence (the CreERT2 sequence modified back to encode the normal Cre recombinase gene), SV40 polyA signal, and FRT flanked neomycin cassette to the ATG start site of the Cartpt locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. The resulting modified BAC was microinjected into embryos from B6C3 mice ((C57BL/6 x C3H)F1 x C57BL/6). Transgenic line 1 (Cart-Tg1-Cre) was established. show more close

HUSBANDRY
Suggested Controls	B6C3F1/J C57BL/6J show more close
Breeding Considerations	When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (non-carrier) siblings, or to C57BL/6J inbred mice. show more close

For Research Use Only.