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STOCK Rai1tm1Luo/J
Cat. No.: CEMM-07250714
Common Name: Rai1-Tag (Rai1Tag)
The Rai1Tag knock-in allele expresses a FLAG/myc-tagged RAI1 (Rai1-Tag) before Cre recombinase exposure. Cre-mediated deletion of the floxed FLAG-myc-STOP sequence results in expression of RAI1/EGFP fusion protein (Rai1EGFP). These Rai1Tag may be useful for studying Rai1 DNA binding, circuit assembly and neuronal communication in human disorders such as Smith-Magenis syndrome (SMS).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Rai1tm1Luo
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Rai1
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| Gene Name |
retinoic acid induced 1
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| Chromosome |
11
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| Expressed Genes |
GFP, Green Fluorescent Protein; FLAG, FLAG octapeptide,
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| MGI Accession ID | show more close |
| Site of Expression |
Prior to Cre recombinase exposure, FLAG/myc-tagged RAI1, consistent with the endogenous gene, is expressed in many cell types in the brain. When bred to mice that express Cre recombinase, EGFP is expressed in in Cre-expressing tissues of the offspring.
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| Strain of Origin |
129S1/Sv
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| Molecular Note |
The targeting vector contains a loxP-flanked FLAG-myc-STOP cassette upstream of an EGFP sequence, all inserted in-frame into the endogenous stop codon. Prior to Cre recombinase exposure, expression of FLAG/myc-tagged RAI1 is observed in a pattern consistent with the endogenous gene, and no EGFP expression is reported.
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| HUSBANDRY | |
|---|---|
| Breeding Considerations |
Mice homozygous for the Rai1Tag allele are viable and fertile with no reported gross physical or behavioral abnormalities.When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice may be bred together.
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For Research Use Only.
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