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B6.Cg-Igs7tm139.1(tetO-EGFP, CAG-tdTomato, -tTA2)Hze/J
Cat. No.: CEMM-07250787
Common Name: Ai139(TIT2L-GFP-ICL-TPT)-D (or Ai139D)
Ai139(TIT2L-GFP-ICL-TPT)-D (also called Ai139D) mice are a Cre-dependent, Tet-controllable dual fluorescent reporter line, created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). Exposure to Cre recombinase removes both STOP cassettes - resulting in EGFP fluorescence, tdTomato fluorescence and tTA2 expression - and that EGFP expression may be diminished/eliminated by doxycycline.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm139.1(tetO-EGFP,CAG-tdTomato,-tTA2)Hze
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| Allele Name |
targeted mutation 139.1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inducible; Transactivator
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| Gene Symbol |
Igs7
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| Gene Name |
intergenic site 7
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| Expressed Genes |
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral; tTA, tetracycline-controlled transactivator, E. coli
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| MGI Accession ID | show more close |
| Site of Expression |
When bred to mice expressing Cre recombinase, the resulting offspring will express EGFP fluorescence, tdTomato fluorescence and tTA2 expression in the Cre-expressing cells/tissues.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The replacement vector is designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element, a Tet response element/promoter (TRE2), a loxP-flanked STOP cassette, a synthetic enhanced green fluorescent protein sequence (EGFP), a woodchuck hepatitis virus post-transcriptional regulatory element, a BGH polyA, two copies of chicken beta-globin HS4 insulator element, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from pCAGGS), a lox2272-flanked STOP cassette, a tdTomato sequence, a viral 2A oligopeptide that mediates ribosomal skipping, a synthetic modified tetracycline-regulated transactivator gene (tTA2S), a WPRE, a BGH polyA, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site.Embryonic stem cells previously targeted with FRT3::AttB::PGK-neoR-polyA::FRT5::splice acceptor::3'hygro cassette::SV40 polyA:AttP in TIGRE, were re-transfected with the pTRE2-LSL-EGFP-CAG-LSL-tdT-P2A-tTA2 replacement vector and Flp recombinase vector for recombinase-mediated cassette exchange (RCME). PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL).
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
The donating investigator reports that heterozygous and homozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice may be bred together.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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