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STOCK Prokr2em1(icre)Cfe/Mmjax

Cat. No.: CEMM-07250891

ProkR2-Cre knock-in mice are designed to have codon optimized iCre recombinase expression directed to prokineticin receptor 2-expressing cells. These mice may be used to generate conditional mutations for studying gonadotropin-releasing hormone neuronal migration, olfactory bulb development, infertility, and Kallmann syndrome.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Prokr2em1(icre)Cfe
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Allele Name
endonuclease-mediated mutation 1
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Allele Attributes
Recombinase-expressing
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Gene Symbol
Prokr2
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Gene Name
prokineticin receptor 2
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Chromosome
2
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Expressed Genes
Cre, Cre recombinase, bacteriophage P1; Prokr2, prokineticin receptor 2, mouse, laboratory
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MGI Accession ID
6119460
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Site of Expression
In females, expression of ProkR2-Cre is found in the medial preoptic area, ventrome-dial nucleus of the hypothalamus, arcuate nucleus, medial amygdala and lateral prabrachial nucleus. In males, expression occurs in the amygdalo-hippocampal area.
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Strain of Origin
(C57BL/6 x SJL/J)F2
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Molecular Note
Using CRISPR/Cas9 technology, a GSG linker, a T2A viral peptide, an iCre recombinase, and a bovine growth hormone polyadenylation signal (bPA) were inserted between the final Prokr2 codon and termination codon. The sgRNA target was cloned into the plasmid pX330. The circular pX330 plasmidandcircular donor plasmid were mixed and injected into the pronuclei of B6SJLF1 fertilized zygotes. Embryos were transferred to pseudopregnant B6D2F1 females to establish the mouse line.
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HUSBANDRY
Breeding Considerations
When maintaining a live colony, these mice may be bred by homozygote sibling matings.
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Breeding Strategy
Heterozygote x Heterozygote
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For Research Use Only.
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