Online Inquiry
C57BL/6J-Gt(ROSA)26Sorem1Naik/J
Cat. No.: CEMM-07251125
Common Name: LoxCode
LoxCode knock-in mice have the LoxCode cassette inserted into the endogenous Gt(ROSA)26Sor locus. The LoxCode cassette is composed of 14 loxP sites in alternating orientation flanking 13 small (8-14nts) code elements. Upon Cre exposure, random recombination between the loxP sites lead to inversions and excisions, resulting in DNA sequence reshuffling with a theoretical DNA diversity of over 30 billion barcodes. This is an inducible, cellular barcoding technology based on the Cre-loxP system that allows for sensitive, high diversity lineage tracing at the cellular, spatial, and tissue level.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sorem1Naik
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| Allele Name |
endonuclease-mediated mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed)
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
C57BL/6J
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| Molecular Note |
Plasmids encoding a guide RNA (CTCCAGTCTTTCTAGAAGAT) are designed to insert the LoxCode cassette into the endogenous Gt(ROSA)26Sor locus. The LoxCode cassette is composed of 14 loxP sites in alternating orientation flanking 13 small (8-14nts) code elements. Upon Cre exposure, random recombination between the loxP sites lead to inversions and excisions, resulting in DNA sequence reshuffling with a theoretical DNA diversity of over 30 billion barcodes.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Although homozygous mice are viable and fertile, the donating investigator suggests breeding heterozygous females with homozygous males when maintaining a colony. First generation homozygous females are fertile with fertility decreasing if colony is kept as homozygous for a few generations.
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For Research Use Only.
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