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B6.Cg-Igs7tm215(CAG-ACR2*, CAG-GCaMP7s)Tasic/J
Cat. No.: CEMM-07251109
Common Name: TICL-stGtACR2-HA-ICL-GCaMP7s-SS
Ai215 (also called Ai215 (TICL-stGtACR2-HA-ICL-GCaMP7s-SS)) is a Cre-dependent optogenetic and fluorescent calcium-indicator tool allele designed with a CAG promoter, loxP-STOP-loxP and stGtACR2 coding sequence followed by a CAG promoter, lox2272-STOP-lox2272 and GCaMP7s coding sequence - all inserted into the Igs7 locus (TIGRE). Exposure to Cre recombinase results in expression of stGtACR2 (soma-targeted variant of the red-shifted, light-gated anion channelrhodopsin GtACR2; neuronal silencing) and/or GCaMP7s fluorescence.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm215(CAG-ACR2*,CAG-GCaMP7s)Tasic
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| Allele Name |
targeted mutation 215
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Epitope tag
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| Gene Symbol |
Igs7
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| Gene Name |
intergenic site 7
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| Chromosome |
9
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| MGI Accession ID | show more close |
| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The targeting vector contains (from 5' to 3') a Bxb1 attL site, a partial green fluorescent protein sequence (non-functional 500bp fragment to inCrease the space between the Bxb1 attB site and the insulator sequence to improve stability of the targeting vector), two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from pCAGGS), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), the coding sequence of stGtACR2 (GtACR2 with targeting sequence TS and Kv2.1 and HA tag sequence), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, the CAG promoter, a lox2272-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-TKpA), the coding sequence of GCaMP7s (with GSG linker sequence and supersoma sequence), a BGH polyA, a PhiC31 attB site, a PGK-5'hygro cassette, an RNA splice donor, a Bxb1 attR site, SA, 3'hygro, SV40pA, and PhiC31 attP. GtACR2 is derived from the Guillardia theta anion channelrhodopsin 2 gene.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Heterozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. To date, it has not been attempted to make this strain homozygous. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice.
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| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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