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STOCK Gt(ROSA)26Sortm1(rtTA, EGFP)Nagy/J
Cat. No.: CEMM-07250027
Common Name: rtTAflox
After breeding with mice carrying Cre recombinase, rtTA and EGFP are expressed in the tissues that express cre in the offspring.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Transactivator
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
rtTA, reverse tetracycline-controlled transactivator, E. coli; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
When these mutant mice are crossed to a strain expressing Cre recombinase, rtTA and EGFP are expressed in the Cre-expressing tissues. These mice can be used to generate mutant mice in which the tissue specificity of Cre-expression and doxycycline inducibility of the rtTA can be combined.
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Upon Cre expression, the floxed neo cassette is removed and the ROSA26 promoter drives expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene.
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| HUSBANDRY | |
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| Breeding Considerations |
When maintaining a live colony, these mice are maintained as homozygotes.
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For Research Use Only.
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