STOCK Et(icre/ERT2)14374Rdav/J

Et(icre/ERT2)14374Rdav

enhancer trap 14374

Recombinase-expressing; Inducible

Et(icre/ERT2)14374Rdav

enhancer trap 14374

UN

cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene

4947570

Cre recombinase activity in brain tissues is found in many cells of the hippocampus (including dentate gyrus projections), with very sparse coverage in all other areas (olfactory bulb, cortex, striatum, thalamus, midbrain, pons, medulla, and hypothalamus).

FVB/N

An enhancer trap lentiviral transgenic approach was used to generate these mice. The transgene was designed with a self-inactivating lentiviral vector backbone (FUGW), a human synapsin minimal promoter (-244 to +47), a beta-globin intron, an iCre /ERT2 fusion (iCre; improved with mammalian codon usage, no putative cryptic splice sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), fused to a mutated human estrogen receptorligand binding domain (ERT2), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability. This packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J mouse embryos. Founders were crossed to C57BL/6 animals. Animals were crossed to B6-Gt(ROSA)26Sortm1Sor mice to assess Cre activity/transmission. Cre positive, lacZ negative mice were subsequently bred to C57BL/6 mice to Create this line.

C57BL/6J Noncarrier

When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (non-carrier) siblings, or to C57BL/6J inbred mice.